Figure 1.

Pretreatment of LNCaP cells with 1α,25(OH)₂D₃ sensitises LNCaP cells to IR-induced apoptosis. (A) Photographs depict representative populations from the different treatment groups at × 100 and × 250 magnifications. Cells with apoptotic morphology appear with brighter stained nuclei (× 100 magnification). In the × 250 magnification, apoptotic cells are indicated by white arrows in the 1,25VD+IR treated group. (B) Cells were treated with ethanol (control) or 100 nM 1α,25(OH)₂D₃ for 48 h prior to treatment with 4 Gy IR and apoptotic cells were counted 12 h after IR. The apoptotic cells from four randomly selected fields were counted (between 50 and150 cells field⁻¹). Three independent experiments were performed. Error bars represent±s.e. values.