Table 2. Comparison of metastases in sentinel nodes (SNs) detected by conventional histology and by reverse transcriptase–polymerase chain reaction (RT–PCR) and Southern blot assay in the six patients with nodal metastases.
|
Metastasis |
||||||
|---|---|---|---|---|---|---|
| Patient no. (location/histological type) | Lymphatic basin | H&E | CK-18 | CEA | hTRT | MUC-1 |
| 1 | L-GA | + | − | − | − | + |
| (Upper/Por) | L-GA | − | − | + | − | + |
| L-GA | − | − | + | − | − | |
| 2 | ||||||
| (Lower/Pap) | R-GEA | + | − | − | + | + |
| R-GEA | − | − | − | + | − | |
| R-GEA | − | − | − | + | + | |
| 3 | R-GEA | + | + | + | − | + |
| (Lower/Sig) | R-GEA | + | + | + | − | − |
| R-GEA | + | + | − | − | + | |
| R-GEA | − | + | + | − | + | |
| R-GEA | − | + | + | − | + | |
| R-GEA | − | − | + | − | − | |
| 4 | L-GEA | + | + | + | − | − |
| (Upper/Tub) | L-GEA | − | + | + | + | − |
| 5 | ||||||
| (Lower/Por) | R-GEA | + | + | + | + | − |
| R-GEA | + | + | + | − | − | |
| R-GEA | − | + | − | + | + | |
| R-GEA | − | + | − | + | − | |
| R-GEA | − | + | − | − | + | |
| R-GEA | − | + | − | − | − | |
| R-GEA | − | − | + | + | − | |
| R-GEA | − | − | − | + | + | |
| R-GEA | − | − | − | + | − | |
| 6 | L-GA | − | + | − | − | − |
| (Upper/Tub) | L-GA | − | + | − | − | − |
Nos. 4 and 5 had metastases in non-SNs detected by conventional histology. No. 6 had metastases in non-SNs detected by immunostaining for CK-18. Pap=papillary adenocarcinoma; Tub=tubular adenocarcinoma; Sig=signet-ring cell carcinoma; por=poorly differentiated adenocarcinoma; L-GA=left gastic artery; L-GEA=left gastroepiploic artery; R-GEA=right gastroepiploic artery; CK-18=cytokeratin-18; CEA=carcinoembryonic antigen; hTRT=human telomerase reverse transcriptase.