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Transcription activation by RhaR T279A in RhaR-CTD expressed alone (RhaR(196-311)). Wild-type RhaR(196-311) or RhaR(196-311) T279A (plasmid-encoded) in strain SME3160 (Φ(rhaS-lacZ)Δ85, Δ(rhaSR)::Km) were assayed for transcription activation of the single copy lacZ fusion. Cultures were grown in minimal glycerol medium with ampicillin and with or without L-rhamnose, as indicated.
