. Author manuscript; available in PMC: 2009 Mar 30.

Published in final edited form as: J Neurosci Methods. 2007 Dec 27;169(1):158–167. doi: 10.1016/j.jneumeth.2007.12.011

Table 2.

Morphology of Map2ab + Cells in Undifferentiated Canine Olfactory Bulb NPC Cultures

Passage	Growth Condition	Map2ab Immunopositivity (%)

		Mature neuronal morphology	Immature small, uni-/bipolar; high N:C ratio
5	PDL^a	1.6 ± 1.6	5.0 ± 1.9
	PDL-fibro^b	0.3 ± 0.2	5.7 ± 1.8
	PDL-LIF^c	3.8 ± 1.1	15.9 ± 3.0

10	PDL	0.0	0.0
	PDL-fibro	0.0	0.0
	PDL-LIF	0.0	11.6 ± 3.7^†

For each experimental variable, values represent the mean ± SEM for duplicate cultures from three isolations.

PDL, poly-D-lysine substrate with standard growth factors;

PDL-fibro, poly-D-lysine plus fibronectin substrate with standard growth factors;

LIF, poly-D-lysine substrate with standard growth factors and leukemia inhibitory factor (LIF).

^†

Value is the mean ± SEM from duplicate cultures of the two isolations that survived.