Table 1.

Kinetic parameters for each enzyme/substrate combination.

Substrate	Enzyme Source	Km	Vmax	CLint
		μM	nmol/min/nmol P450	ml/min/nmol P450
Diclofenac	Baculosomes®	5.1 ± 0.9	29 ± 2	5.7
	Supersomes™	2.6 ± 0.3	14.8 ± 0.4	5.7
	RECO®	16 ± 2	16.8 ± 0.9	1.1
	Reconstituted	30 ± 3	27 ± 1	0.9
	HLMc	4.0	1670a	420b
(S)-Flurbiprofen	Baculosomes®	16.2 ± 0.9	34 ± 1	2.1
	Supersomes™	21.6 ± 0.8	11.7 ± 0.8	0.54
	RECO®	120 ± 10	16.8 ± 0.5	0.14
	Reconstituted	45 ± 2	11.5 ± 0.2	0.25
	HLMd	1.9	343 a	180 b
Tolbutamide	Baculosomes®	103 ± 6	10.1 ± 0.2	0.098
	Supersomes™	194 ± 29	4.0 ± 0.2	0.021
	RECO®	1462 ± 50	5.6 ± 0.1	0.0038
	Reconstituted	220 ± 19	3.9 ± 0.1	0.018
	HLMc	147	276 a	1.88 b
(S)-Warfarin	Baculosomes®	4.6 ± 0.3	2.33 ± 0.03	0.51
	Supersomes™	13 ± 2	0.38 ± 0.01	0.029
	RECO®	32 ± 2	0.698 ± 0.009	0.022
	Reconstituted	52 ± 9	1.54 ± 0.07	0.030
	HLMe	3.7	10.5a	2.8 b

^aUnits of pmol/min/mg protein

^bUnits of μl/min/mg protein

^c(Walsky and Obach, 2004)

^d(Tracy et al., 1995)

^e(Hemeryck et al., 1999)