Table 1. Percentage of cells expressing each cell cycle marker in normal vulval epithelium and VIN 1, VIN 2 and VIN 3.
| Normal (n=6) | VIN 1 (n=3) | VIN 2 (n=8) | VIN 3 (n=20) | Correlation coefficient | |
|---|---|---|---|---|---|
| Mcm 2 | 14.7 (9.9–19.4) | 32.3 (19.2–52.5) | 85.0 (80.5–94.1) | 93.4 (82.9–97.8) | rs=+0.91 |
| P<0.001 | |||||
| Mcm 5 | 16.3 (9.7–21.7) | 44.2 (33.6–61.0) | 85.1 (79.6–91.7) | 94.1 (84.5–98.9) | rs=+0.90 |
| P<0.001 | |||||
| Cyclin B1 | 1.9 (0.6–3.9) | 2.5 (1.1–4.8) | 9.5 (6.0–13.6) | 9.7 (3.4–16.2) | rs=+0.68 |
| P<0.001 | |||||
| Phospho-H3 | 2.7 (0.5–4.7) | 3.4 (1.0–7.3) | 7.2 (1.8–14.1) | 10.0 (3.0–26.2) | rs=+0.65 |
| P<0.001 | |||||
| In situ DNA replication | 1.6 (1.8–3.4) | 3.4 (2.0–5.3) | 8.1 (4.7–12.3) | 13.4 (5.5–30.2) | rs=+0.81 |
| P<0.001 |
This table shows the mean labelling indices (percentage of stained cells) for normal vulval epithelium and VIN 1, VIN 2 and VIN 3 with each of the cell cycle markers. Similar patterns of staining were observed for cyclins B1 and D1, but precise quantification of the latter was not possible. Ranges are given in parentheses. The proportion of positively stained nuclei increases with the level of dysplasia with each of the markers studied. The Spearman rank correlation coefficients and level of statistical significance are shown.