Figure 5.

Effects of GRGDS peptide on adhesion of α5B2 to the glomerulus in the kidney section. (A) Laser microscopic appearance of adhesion assay on the kidney section. 10⁵ cells/section were incubated for 30 min with gentle rotation. a, CHO-B2 cells (yellow spots), α5-defective mutant, slightly adhere to the glomerulus (red spots). b, α5B2 cells which were α5 transfectants effectively adhered to the glomerulus. c, Adhesion of α5B2 cells to the glomerulus was not changed by administration of 200 μg ml⁻¹ GRGES peptide. d, Adhesion of α5B2 cells to the glomerulus was inhibited by administration of 200 μg ml⁻¹ GRGDS peptide. (B) Cell number per glomerulus (n=20). Note that adhesion of α5B2 cells with GRGDS treatment to the glomerulus was significantly inhibited as compared with GRGES treatment (^*P<0.0001).