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. 2003 Jan 28;88(2):320–326. doi: 10.1038/sj.bjc.6600679

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Copyright © 2003 Cancer Research UK

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Signal transduction molecules. The percentage of positive lymphocytes was evaluated before (TIL-1) and after (TIL-2) culture with IL-2. TCR, ζ and ɛ chains and p56^lck expression was evaluated in TIL-1 by IHC staining on paraffin-embedded surgically removed specimens. The same molecules were evaluated in TIL-2 by IHC staining on cytocentrifuged slides. The assays were performed on 20 melanoma and 16 colon carcinoma samples.