Table 2.

Proteins Containing BIAM-Reactive Thiol Groups

Spot Number	Protein identification	MOWSE	MW (kDa)
1	heat shock protein 70 kDa	246	72.3
2	ND
3	Succinyl- CoA Ligase Beta Chain and Ubiquinol- cytochrome-c reductase complex core protein I	205	46.8 & 52.7
4	Hspd1 Protein	186	59.4
5	Albumin	96	69.3
6	Enoyl Coenzyme A hydratase 1, peroxisomal	75	36.1
7	sterol carrier protein 2, liver	88	59.0
8	Sdha protein	109	72.2
9	ND
10	3-hydroxy-3-rnethylglutaryl-coenzyme A synthase 2	80	56.8
11	Catalase	135	59.7
12	3-hydroxy-3-rnethylglutaryl-coenzyme A synthase 2	105	56.8
13	Catalase	85	59.7
14	Catalase	180	59.7
15	Hydroxyacid oxidase 1	125	40.9
16	ND
17	carnitine palmitoyltransferase 2	106	73.8
18	acetyl-coenzyme A acyltransferase 2 (mitochondrial 3-oxoacyl-coenzyme A thiolase)	144	41.8

Proteins listed above were matched on all immunoblots and gels from the control, 1, 3, and 6 h exposure to APAP. Thiol content in protein spot 12, HMG-CoA synthase, was shown to be significantly altered by APAP treatment (Figure 5). Spot numbers are the same as those used to identify BIAM labeled proteins spots in Figure 4A and B. ND = no designation, MOWSE is an algorithmic calculation used to assign a statistical weight to each peptide match; therefore the higher MOWSE score implies higher statistical weight of a match.