Figure 1.

Apoptosis induction and change in HO activity after treatment with ZnPP IX of AH136B solid. Apoptosis (A, B) and HO activity (C) were assessed on day 14 after tumour implantation. Each specimen was analysed via TUNEL staining 24 h after treatment with vehicle (the control) (A, a), with ZnPP IX (500 μg kg⁻¹ i.a.) (A, b), or with CuPP IX (500 μg kg⁻¹ i.a.) (A, c). Quantitative analysis of TUNEL-positive cells in each specimen is shown (B). TUNEL-positive cells were counted in four different fields of magnification at × 100 per sample, and then the number of positive cells per mm² was calculated. Haem oxygenase activity of the solid tumour was measured after treatment in the same manner as in the TUNEL analysis (C). ^**P<0.05, ^**P<0.01 vs control (n=3 for each group). Data are means±s.e. See text for details.