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. 2003 Mar 18;88(6):937–939. doi: 10.1038/sj.bjc.6600751

Figure 2.

Figure 2

A33scFv–CD-mediated cytotoxicity on A33 antigen-positive vs negative cells: LIM1215 cells and HT29 cells were incubated with a dilution series of A33scFv–CD fusion protein and, after washing, with the 5-FC prodrug. Survival was measured by the MTT method as described. A33scFv–CD fusion protein from two different preparations was used on HT29 cells (▴ and ▾) and on LIM1215 cells (• and ⧫). As a control, a single, high concentration of A33scFv–GFP (□) was used instead of A33scFv–CD. Mean and s.d. of triplicate samples.