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. 2003 Mar 18;88(6):937–939. doi: 10.1038/sj.bjc.6600751

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Copyright © 2003 Cancer Research UK

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MTT cytotoxicity blocking assay. As a negative control, A33scFv–CD was used without subsequent prodrug incubation (▪), and 5-FU alone served as positive control (▴). In the complete ADEPT assay with subsequent 5-FC incubation as described in the text, cells were preincubated either with the “A33scFv-GFP” antibody (⧫) or with hu3S193 as an isotypic control antibody (▾) for 1 h before the fusion protein was added. Mean and s.d. of triplicate samples.