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. 2003 Mar 18;88(6):871–878. doi: 10.1038/sj.bjc.6600802

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Copyright © 2003 Cancer Research UK

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Fibulin-1 expression and processing in ER/PR-positive and ER/PR-negative human breast tumour-derived cell lines. In (A) and (B), aliquots of concentrated CCM and detergent extracts of the cells, respectively, were immunoblotted using antifibulin-1 mAb. In (C), blot B was stripped and reprobed with an anti-PR mAb that detects two isoforms of the PR protein, PRB (120 kDa) and PRA (94 kDa). Only the ER/PR-positive cell lines expressed detectable levels of PR protein. In (D), blot B was again stripped and reprobed with the antiactin mAb.