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. 2003 Mar 18;88(6):871–878. doi: 10.1038/sj.bjc.6600802

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Copyright © 2003 Cancer Research UK

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Western blot analysis of the effect of oestradiol (E₂) on fibulin-1 expression. Purified fibulin-1 protein (lane 1) and aliquots of concentrated CCM (10 ml original volume) from either steroid-depleted serum-free medium containing ethanol vehicle (lane 2) or steroid-depleted serum-free medium containing 10 nM exogenous E₂ (lane 3) were immunoblotted using antifibulin-1 3A11 mAb. See Materials and Methods for details of culture conditions. Blot is representative of three independent experiments.