Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 May 13;88(10):1560–1565. doi: 10.1038/sj.bjc.6600940

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Bisulphite sequencing of cyclin D2 promoter region. The nucleotide sequence from −1220 to −883 of the cyclin D2 gene is shown. The individual CpG sites between two PCR primers are numbered sequentially. Cytosines at the CpG site are in capitalisation. The bisulphite sequencing PCR primers are bold and underlined whereas the MSP primers are shown as italic. DNA from five gastric cell lines, two cyclin D2-positive cancers (T2, T39), two cancers with low cyclin D2 expression (T8, T39) and three cyclin D2-negative (T4, T6, T30) cancers as well as one normal gastric tissue (N1) were bisulphite-treated, PCR-amplified and subcloned. The sequencing results from five to eight clones for each cell line and samples are presented. Each horizontal line represents the sequencing result of one subclone. CpG sites within 48 bp are shown as one block. Methylated CpG sites are shown as ‘•’ whereas ‘○’ indicate unmethylated CpG sites.