Figure 7.

Western blot analysis of apoptosis-regulating proteins in clearCa-22 after exposure to TRAIL (10 ng ml⁻¹) or IR (1 Gy) alone and in combination for 12 h. Reduced expression of c-FLIP after exposure to TRAIL and/or IR, whereas expression of FADD and survivin remained unchanged when compared with the control (0). No cleavage of Bid into tBid and absence of active phosphorylated Akt protein after exposure to TRAIL and/or IR. High levels of XIAP protein (53 kDa) before and after exposure to TRAIL and/or IR and appearance of an XIAP cleavage product after exposure to TRAIL and/or IR. (Cleavage of Bid into tBid in J16 cells after exposure to CH11 was used as a positive control; expression of phosphorylated Akt in PDGF-induced NIH-3T3 cells was used as a positive control, as recommended by the manufacturer).