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. 2003 Feb 18;88(4):599–605. doi: 10.1038/sj.bjc.6600722

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Copyright © 2003 Cancer Research UK

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DNA adduct profiles in (A) MCF-7 and (B) AH^R100 cells. Cells were incubated with (i) 0.1% DMSO or (ii) 1 μM 5F 203 for 24 h. ³²P-labelled adducts were analysed using 2 μg digested DNA on a Hypersil C18 column with a gradient of acetonitrile in 2 M ammonium formate as detailed in Materials and Methods. The early peak is due to the presence of free [γ-³²P]ATP.