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. 2003 Feb 18;88(4):599–605. doi: 10.1038/sj.bjc.6600722

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Copyright © 2003 Cancer Research UK

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5F 203 induces accumulation of cells in S phase. Exponentially growing cells (MCF-7 and AH^R100) were exposed to either 0.1% DMSO (control) or 5F 203 (1 μM) for 24 h, harvested, washed in PBS, and fixed in 70% ethanol. DNA was stained by incubating the cells in PBS containing propidium iodide and fluorescence measured and analysed as described in Materials and Methods.