Figure 1.

Identification of human lung cancer cells transfected with gelsolin. (A) Construction of gelsolin-overexpressive clones. The human cytoplasmic gelsolin cDNA was cloned into the HindIII–StuI site of pLNCX retoroviral vector as described in Materials and Methods. The resulting construct, pLNChGsn or pLNCX alone was transfected into CAK8, a highly efficient ecotropic virus-packaging cell line. (B) Western blot analysis of the gelsolin expression in stably transfected clones. PC10 cells were transfected with pLNCX or pLNChGsn, and stable transfectants were established after selection with G418 as described in Materials and Methods. Parental (lanes 1), neo-transfectants (lanes 2 and 3) and gelsolin transfectants (lanes 4 and 5) are shown. (C) Densitometric quantification of protein levels in transfectants is shown after normalisation against the levels of actin protein using NIH Image.