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. 2008 May 15;22(10):1345–1355. doi: 10.1101/gad.470008

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Figure 3. — RBP2 interacts with the PRC2 complex. (A) IP assays using 293T cell lysates expressing HA-tagged wild-type (WT) or enzymatically inactive RBP2 mutant showing specific coimmunoprecipitation of RBP2 with EZH2 and EED. (B) IPs of mouse ES cell lysates using specific antibodies to Ezh2 and Suz12 showing specific coimmunoprecipitation of Rbp2. IP with anti-HA-tag antibody served as negative control. (C, top panels) Size exclusion chromatography (Superose-6, GE Healthcare) of mouse ES cell protein lysate showing coelution of Rbp2, Ezh2, Suz12, and Eed in high-molecular-weight fractions. (Bottom panels) The indicated fractions were immunoprecipitated with a Suz12-specific antibody. IP with anti-HA-tag antibody served as negative control (Ctrl). (D) Coomassie-stained gel and Western blot of protein-immunoprecipitated material from Sf9 cells expressing recombinant RBP2, EZH2, SUZ12, and Eed with anti-SUZ12 and anti-HA-tag (as negative control) antibodies showing the binding of RBP2 to the PRC2 complex.