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. 2008 May 15;22(10):1369–1380. doi: 10.1101/gad.1654308

Figure 3.

Figure 3.

Mutation of RAT1 and XRN1 suppresses the temperature-sensitive phenotype of trm8-Δ trm4-Δ mutants. (A) Schematic of the role of Met22 in the sulfate assimilation pathway. (B) The temperature-sensitive phenotype of trm8-Δ trm4-Δ mutants is suppressed on media containing LiCl. Wild-type and trm8-Δ trm4-Δ strains were grown in YPD at 28°C, plated as in Figure 1 on SD media containing 0.2M LiCl or 1M KCl, and incubated at 30°C or 37°C. (C) RAT1 complements suppressors of trm8-Δ trm4-Δ strain. trm8-Δ trm4-Δ suppressor strains and controls, transformed with a URA3 CEN RAT1 plasmid or a vector control, were grown at 28°C in SD-Uracil media, plated on SD-Uracil media, and incubated at temperatures indicated. (D) Mutation of both RAT1 and XRN1 improves suppression of the trm8-Δ trm4-Δ temperature-sensitive phenotype. Strains were grown in YPD at 28°C and plated on YPD media at indicated temperatures.