Figure 5.
Transcription inhibition does not affect tRNAVal(AAC) levels in a trm8-Δ trm4-Δ rat1-107 xrn1-Δ strain. (A) Northern blot analysis of tRNA after treatment with thiolutin. Strains were grown at 28°C to OD600 ∼ 1.5, treated with 5 μg/mL thiolutin for 10 min, and then shifted to 37°C for indicated times before harvest, RNA preparation, and analysis of 5 μg of RNA by Northern blotting. (B) Quantification of the levels of tRNAVal(AAC). Thiolutin-treated samples are indicated by dashed lines and open symbols, and untreated samples are indicated by solid lines and closed symbols. Wild type is indicated by squares, trm8-Δ trm4-Δ is indicated by circles, and trm8-Δ trm4-Δ rat1-107 xrn1-Δ is indicated by triangles.