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Deletion of MET22 suppresses the temperature-sensitive phenotypes of multiple strains lacking different sets of tRNA modifications. (A) A met22-Δ mutation rescues growth of trm8-Δ pus7-Δ and trm8-Δ dus3-Δ mutants at high temperature. Strains were grown at 28°C and plated on YPD media at indicated temperatures. (B) A met22-Δ mutation or a rat1-107 mutation rescues growth of trm44-Δ tan1-Δ mutants at high temperature. Strains were grown and plated as in A.
