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. 2008 May 15;22(10):1369–1380. doi: 10.1101/gad.1654308

Figure 8.

Figure 8.

Mature tRNASer(CGA), tRNASer(UGA), and tRNALeu(GAG) are degraded in the trm44-Δ tan1-Δ strain. (A) Northern blot analysis of tRNA in wild-type and trm44-Δ tan1-Δ strains after thiolutin treatment. Strains were grown in YP glycerol at 30°C to OD600 ∼ 0.16, treated with 5 μg/mL thiolutin (TL) for 5 min at 30°C, and shifted to 36.5°C for indicated times. Two micrograms of RNA were analyzed by Northern blotting. (B,C) Quantification of the levels of tRNASer(CGA) and tRNASer(UGA). tRNA levels quantified as described in Figure 2.