Figure 1.

Purification and identification of CtBP-1 and CtBP-2 in the native PRDM16 transcriptional complex. (A) Silver staining of the PRDM16-associated proteins. Nuclear extracts prepared from adipocytes expressing either vector (left lane) or PRDM16 (right lane) were purified using Flag M2 agarose. After extensive washing, the PRDM16 complex was eluted with 3xFlag peptide and separated by 4%–12% gradient SDS-PAGE. (B) PRDM16 complex was immunopurified from the immortalized brown fat cells and separated by SDS-PAGE. Endogenous CtBP-1 and CtBP-2 were detected by Western blotting.