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Correlation between measured necrotic fraction and predicted necrotic fraction based on the samples metabolic profile (r = 0.96 p < 0.001 for training and r = 0.76, p < 0.002 for validation) (A). Score plot of PC1 versus PC2 from PLS calibration of necrotic fraction to single-pulse spectral data for untreated and irradiated HT29 xenografts (B) and the corresponding loading profile of PC1 (C).
