Skip to main content
. Author manuscript; available in PMC: 2008 May 13.
Published in final edited form as: Nat Neurosci. 2005 May 22;8(6):723–729. doi: 10.1038/nn1473

Figure 7.

Figure 7

Neurospheres derived from a single stem cell can generate neurons and glia in vivo. Prominin+lin- cells from actin-CFP transgenic mice were cultured at clonal density for 10 d to generate neurospheres. Neurospheres were injected into the cerebellum of 3-d-old mice (one neurosphere per host). After 2-3 weeks, host cerebella were fixed and stained with antibodies specific for S100β (a), NG2 (b), TuJ1 (c), GABAA receptor α6(g), parvalbumin (h) or calbindin (i). These antibodies were detected with TRITCconjugated secondary antibodies (red). Chicken antibodies to GFP and FITCconjugated anti-chicken antiserum were used to amplify the CFP signal (green). Double-labeled cells (orange-yellow in a-c and g-i) were identified and photographed at 40× magnification; 10 bright-field pictures (d-f and j-l) indicate the location of transplanted cells×in the corresponding fluorescent panels. WM, white matter; pia, pial membrane surrounding surface of cerebellum; ML, molecular layer; IGL, internal granule layer; PCL, Purkinje cell layer.