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. Author manuscript; available in PMC: 2008 Oct 10.
Published in final edited form as: Nature. 2008 Mar 12;452(7188):713–718. doi: 10.1038/nature06731

Figure 1. ATXN1-S776D but not ATXN1-S776A interacts with RBM17.

Figure 1

(a) Schematic representation of the ATXN1 constructs.

(b) RBM17 specifically interacted with ATXN1-S776D in the Y2H screen. AD (activation domain) and DB (DNA binding domain) of Gal4 were fused to human ATXN1 or RBM17, respectively. Y2H controls are: lane 1, negative control; lane 2, weak positive control; and lanes 3–5, strong positive controls.

(c) ATXN1 interacted with RBM17 in HEK293T cells by co-AP assays. Top panel shows expression of myc-RBM17 after affinity purification on Glutathione-Sepharose 4B beads, demonstrating the ATXN1/RBM17 interaction (arrow). GST-empty vector was used as a control (−). IB, Immunoblot.

(d) Co-IP of Atxn1 with RBM17 from wild-type mouse cerebellar extracts. The anti-RBM17 antibody co-immunoprecipitated Atxn1 (arrow), but not the long and short isoforms of Capicua, CIC-L and CIC-S, respectively (bracket).