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. Author manuscript; available in PMC: 2008 Oct 10.
Published in final edited form as: Nature. 2008 Mar 12;452(7188):713–718. doi: 10.1038/nature06731

Figure 2. Enhanced interaction of RBM17 and ATXN1 depends on S776-phosphorylation and polyglutamine tract expansion.

Figure 2

(a) Wild-type ATXN1 and ATXN1-S776D, but not ATXN1-S776A, interacted with RBM17.

(b) RBM17 bound most strongly to polyglutamine-expanded ATXN1. A bracket showed that there is no obvious difference in S776-phosphorylation depending on polyglutamine expansion in ATXN1 when using a PN1168 antibody (specific to phosphorylated S776 of ATXN1) for IB.

(c,d) RBM17 expression in the nuclei of PCs of 18-week-old mice from either wild-type (FVB)

(c) or SCA1 transgenic (ATXN1[82Q]) homozygotes (d). RBM17 protein (green) was co-immunostained with calbindin (red, PC marker) and Toto3 (blue, nuclear marker).