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EM micrographs of shadowed (a–c) and negatively stained (d) single double-row-type polysomes isolated by glycerol gradient centrifugation. Polysomes were isolated after 2 h of translation of luciferase mRNA in the wheat germ CECF system. Fractions of heavy (a,b,d) and medium-size (c) polysomes are presented.
