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. 2008 Mar 19;36(8):2690–2699. doi: 10.1093/nar/gkn032

Figure 3.

Figure 3.

Smad3 is required for the transcriptional inhibition of miR-24 by TGF-β1. (A) Expression of Smad3 detected by Western blot in Smad3–/– primary myoblast cells. β-actin was used as a loading control. (B) Enhanced expression of miR-24 in Smad3–/– primary myoblast cells in both GM and DM for 2 days. U6 RNAs detected by Northern blot were used as loading controls. (C) Enhanced expression of differentiation markers (MEF2d, Myogenin and MHC) in Smad3–/– primary myoblast cells in both GM and DM. β-actin was used as a loading control. (D) The expression of miR-24 expression in Smad3+/+ and Smad3–/– primary myoblast cells transferred into DM with or without TGF-β1 (5 ng/ml) for 2 days. (E) Expression of differentiation markers (MEF2d, Myogenin and MHC) in Smad3–/– primary myoblast cells transferred into DM with or without TGF-β1 (5 ng/ml) for 2 days.