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. 2008 Mar 26;36(8):2728–2738. doi: 10.1093/nar/gkn028

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Iodine probing of AatRNA^Leu_GAG containing phosphorothioate nucleotides. (A) AatRNA^Leu_GAG was labeled at its 5′-end and probed in the presence (+) or absence (−) of AaLeuRS; Ctrl is the control without iodine. (B) Cloverleaf structure of AatRNA summarizing the protections observed in the tRNA following AaLeuRS binding. Three intensities of protections are shown. (C) View of the T. thermophilus LeuRS/tRNA^Leu complex (2BYT) (10). The enzyme is shown in red and the tRNA is in blue. The tRNA nucleotides protected from iodine cleavage in AatRNA are highlighted in yellow.