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. 2008 Mar 26;36(8):2728–2738. doi: 10.1093/nar/gkn028

Figure 5.

Figure 5.

Effect of AatRNALeuGAG mutations on the editing reactions of AaLeuRS. In (A)–(C), different colors represent various AatRNALeuGAG variants: AatRNALeuGAG (wild-type), black; AatRNALeuGAG (A35U), red; AatRNALeuGAG (A35C), green; AatRNALeuGAG (A35G), yellow; AatRNALeuGAG (ΔAC), blue; and AatRNALeuGAG (C41U), pink. Cyan curve shows the background rate in the ATP consumption assay in the absence of isoleucine in (A) or the spontaneous hydrolysis in the absence of the enzyme in (B). (A) ATP consumption during total editing by AaLeuRS in the presence of 50 mM isoleucine and 5 μM AatRNALeu mutant transcripts. (B) Hydrolysis of Ile-tRNALeuGAG for AatRNALeuGAG mutants by 5 nM AaLeuRS. The spontaneous hydrolysis in the absence of the enzyme (<10%) were subtracted. (C) Isoleucylation rates of 5 μM AatRNALeu mutants by 1 μM LeuRS. Data are averaged from three independent assays.