. 2008 Mar 26;36(8):2728–2738. doi: 10.1093/nar/gkn028

Table 1.

Kinetic constants of AaLeuRS for AatRNA^Leu_GAG and derived mutants in the aminoacylation reaction

tRNA domain	Mutants of AatRNA	k_cat (s⁻¹)	K_m (μM)	k_cat/K_m (μM⁻¹s⁻¹)	k_cat/K_m (relative)
	native	1.28 ± 0.34	1.10 ± 0.26	1.16	1.0
Acceptor-arm	A73G	0.06 ± 0.015	7.40 ± 2.6	0.008	0.007
	A73U	0.19 ± 0.05	7.65 ± 1.4	0.025	0.022
	A73C	0.15 ± 0.06	6.29 ± 2.1	0.024	0.021
	A5:U68&G6:C67	1.46 ± 0.41	1.60 ± 0.45	0.91	0.78
	A4:U69&A5:U68	1.67 ± 0.33	0.83 ± 0.21	2.01	1.73
	C69U	0.098 ± 0.014	3.29 ± 0.45	0.03	0.026
D-arm	G13C	0.56 ± 0.08	0.32 ± 0.056	1.75	1.51
	A14U	0.53 ± 0.12	18.00 ± 2.7	0.029	0.025
	A15U	1.13 ± 0.15	1.21 ± 0.18	0.93	0.8
	C16G	1.34 ± 0.31	1.05 ± 0.12	1.28	1.10
	U17A	1.03 ± 0.26	1.18 ± 0.3	0.87	0.75
	G18C	0.56 ± 0.062	5.20 ± 0.87	0.11	0.095
	G19C	nm	nm	nm	nm
	C20A	1.21 ± 0.17	1.50 ± 0.25	0.81	0.70
	A20aC	0.94 ± 0.14	2.19 ± 0.38	0.43	0.37
	G21A	0.59 ± 0.077	1.37 ± 0.22	0.43	0.37
	G13:C23	1.061 ± 0.32	3.76 ± 0.63	0.28	0.24
	G19U:C56A	0.94 ± 0.13	1.49 ± 0.14	0.63	0.54
Anticodon-arm	A35U	1.52 ± 0.22	1.60 ± 0.21	0.95	0.82
	A35C	1.58 ± 0.37	0.94 ± 0.16	1.68	1.45
	A35G	1.34 ± 0.24	0.97 ± 0.21	1.38	1.19
	Δ35-37	1.52 ± 0.32	0.77 ± 0.11	1.97	1.70
	ΔG29:C41	1.01 ± 0.21	0.89 ± 0.092	1.13	0.97
	C41U	0.84 ± 0.12	0.85 ± 0.23	0.99	0.85
	ΔAC	1.43 ± 0.19	2.77 ± 0.31	0.52	0.45
Variable-arm	V-arm^Ser	0.11 ± 0.026	4.03 ± 0.55	0.027	0.023
	V-arm-2bp	0.75 ± 0.15	1.42 ± 0.26	0.53	0.46
	ΔV-arm	0.38 ± 0.05	4.20 ± 0.57	0.09	0.078
	U48A	0.64 ± 0.082	3.64 ± 0.53	0.18	0.16
	ΔG47k	0.48 ± 0.05	4.02 ± 0.61	0.12	0.10
	ΔU48	nm	nm	nm	nm
	A47eU	1.41 ± 0.15	0.85 ± 0.076	1.66	1.43
TψC-arm	ΔC59	nm	nm	nm	nm
	A58G	1.57 ± 0.41	9.16 ± 1.86	0.17	0.15

All kinetic data were reproduced at least 3 times; nm: not measurable.