Table 4.
Variant | Relative kcat/KM (±SD) | Activity change (x-fold) | −ΔΔG‡ (kcal/mol) | (−ΔΔG‡)Me a (kcal/mol) |
---|---|---|---|---|
WT | 1.00 | 1.0 | 0.0 | 0.0 |
dC75Me | 0.28 (±0.05) | −3.6 | 0.76 | −0.41 |
dC74Me | 0.23 (±0.05) | −4.3 | 0.87 | −0.19 |
dC73Me | 0.14 (±0.07) | −7.3 | 1.17 | −0.30 |
dC72Me | 0.11 (±0.03) | −8.8 | 1.29 | 0.55 |
dC71Me | 0.05 (±0.02) | −18.5 | 1.73 | 1.66 |
dG70Me | 0.06 (±0.03) | −17.0 | 1.68 | 1.50 |
dA69Me | 0.77 (±0.05) | −1.3 | 0.16 | −0.13 |
dG68Me | 0.80 (±0.04) | −1.2 | 0.13 | −0.35 |
dC67Me | 0.16 (±0.03) | −6.2 | 1.08 | 0.40 |
dA66Me | 0.13 (±0.08) | −7.8 | 1.22 | −0.51 |
dG65Me | 0.29 (±0.15) | −3.5 | 0.74 | −0.18 |
dG64Me | 0.38 (±0.12) | −2.6 | 0.57 | −0.26 |
dC63Me | 0.26 (±0.07) | −3.8 | 0.80 | 0.20 |