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. 2008 Mar 29;36(8):2787–2798. doi: 10.1093/nar/gkn144

Figure 2.

Figure 2.

BBP–Mud2p purifies as a heterodimer. (A) HPLC resolution of molecular weight standards by sequential 2000Å and 300Å sepharose columns. (B) Western blots of the BBP–TAP and Mud2–TAP selected on calmodulin agarose and resolved by HPLC. The blots were developed with an antibody against the TAP epitope. T, total protein applied to the tandem size exclusion columns. The sample numbers correspond to the same column fractions indicated in (A).