Figure 3.

Transient transactivation of CAT reporter via binding to Egr1 EBS by ETS1 and FLI1 proteins. (A) Map of the CAT reporter plasmids. The pBLCAT2 was used to construct reporter plasmid that contained the Egr1 SREI. (B) CAT assays were carried out with plasmids containing CAT reporter gene linked to Egr1 SREI and either ETS1 or FLI1 expression vectors. The CAT activity was examined by thin layer chromatography. The reaction products were quantified by analyzing the TLC plates on an AMBIS radioisotopic imaging system.