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Construction of pPC1 expression plasmid. (A) Plasmid pBluMH. The thin line indicates the plasmid region derived from pBluescript vector, the empty box indicates the probe area. Number in parenthesis indicates the length of DNA (in kb) with reference to the PstI site near T3 promoter as the origin point. MHPCO gene (B) was isolated from pBluMH by PCR and ligated to pET-11a (C), resulting in the expression plasmid pPC1 (D). The mark indicates the length representing 1,000 nucleotides.
