Figure 1.

Synergistic stimulation of DNA synthesis of hepatocytes in primary culture by the LMW and HMW factors derived from the bovine small intestinal mucosa. The LMW factor (3%, vol/vol) and the HMW factor (1%, vol/vol) from the bovine small intestinal mucosa slightly stimulated DNA synthesis ([³H]thymidine uptake) when each of them was independently added to rat hepatocytes in primary culture. However, a synergistic increase in stimulation was seen when they were added simultaneously. The LMW factor and the HMW factor are referred to as LMW and HMW, respectively. All bars are the mean of duplicate assays.