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Localization of EEA1 in 3T3-L1 adipocytes. Cells were serum-deprived for 3 hr, treated for 15 min without (Left) or with (Right) wortmannin (20 nM), fixed and double-stained with antibodies to EEA1 (Upper) and GLUT4 (Lower). Images were collected using a cooled charged-coupled device camera. Equivalent exposures are shown.
