Figure 2.
Expression of an epithelial cell marker (DPPIV), a liver-specific gene (albumin), and a pancreatic/biliary ductal cell marker (CK-19) in cell fractions isolated from the Cu-deficient rat pancreas. Cell fractions 1–4 collected at different densities in a discontinuous Nycodenz gradient were obtained as noted in Materials and Methods. Fractions 1–4 are displayed from left to right. A–D are stained histochemically for DPPIV (×100), E–H are hybridized for albumin mRNA (×200) using a 35S-labeled antisense albumin RNA riboprobe as previously reported (7), and I–L are immunohistochemically stained for CK-19 (×100) using a monoclonal anti-CK-19 as primary antibody (Amersham) and polyvalent anti-mouse immunoglobulin peroxidase conjugate (Sigma) as secondary antibody.