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. 1997 Jul 8;94(14):7378–7383. doi: 10.1073/pnas.94.14.7378

Figure 1.

Figure 1

PCR with the mouse-specific polymorphic markers D18Mit64 and D18Mit146 to confirm the presence of the mouse NP-C segment in fused cell lines. The lane order is the same in the left and right halves. Lanes: 1, ELN; 2, ELC; 3, CT60 neoR HATS; 4–7, CT60 neoR HATS fused to lanes 4 and 5 (ELN) and lanes 6 and 7 (ELC); 8, H2O. Middle lane (size marker, M): λ/HindIII fragments plus øx 174 RF DNA/HaeIII fragments. Arrowheads indicate the expected size PCR products (D18Mit64, 172 bp; D18Mit146, 138 bp). Clone identification numbers are indicated in parentheses.