Figure 2.

Characterization of circular BRCA2 YACs obtained by TAR cloning from total human DNA. Chromosomal-size DNA was isolated from transformants containing BRCA2 YACs, exposed to a low dose of γ rays (5 Krad), separated by TAFE gel electrophoresis, and blot hybridized with total human genomic DNA (6). The strong signals at the positions of the starting wells correspond to large circular molecules. The leading bands at approximately 97 kb correspond to molecules linearized by the radiation. Four subclones of isolates 27 and 32 (lanes 1–4 and 5–8, respectively) and two subclones of isolate 60 (lanes 9 and 10) were analyzed.