Figure 3.

Alu-profile characterization of YACs containing BRCA2. Subclones of the three initial isolates were characterized and compared in terms of Alu profiles of restriction fragments. Total yeast DNA was isolated from each subclone and digested to completion with TaqI. Fragments were separated by gel electrophoresis, transferred to a nylon membrane, and hybridized with an Alu probe. Four subclones of isolates 27 (lanes 1–4) and 32 (lanes 5–8) and three subclones of isolate 60 (lanes 9–11) were analyzed.