Figure 4.

PCR analysis of YACs containing BRCA2. The presence of BRCA2 coding region in the YAC clones was examined by PCR using 26 pairs of exon primers and 2 pairs of junction primers. Presented in this figure are PCR products obtained for exons 3, 11, and 25 and for the 5′ and 3′ TAR vector junction sequences. (We note that comparable results were obtained for the other primers, in that the PCR products with the YACs matched those of the total human DNA.) The first three lanes for each set of PCR primers correspond to products obtained from the three independent BRCA2 YAC isolates. The fourth lane corresponds to the product generated from total human DNA. (In the PCR analysis of the 3′ TAR junction some additional bands were observed with the total human DNA that were due to PCR artifacts.) The fifth lane was a control in which no DNA was added (there was no fifth lane for the 3′ TAR junction). The first and last lanes contained 123-bp ladders.