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. Author manuscript; available in PMC: 2008 May 13.
Published in final edited form as: Am J Physiol Heart Circ Physiol. 2007 Jan 12;292(4):H1664–H1674. doi: 10.1152/ajpheart.01138.2006

Fig. 6.

Fig. 6

A: representative images of the effects of inhibitors of NADPH oxidase, phosphatidylinositol 3-kinase (PI3K), and ERK1/2 on H/R-stimulated sprouting from PCAEC spheroids at 24 h. B: quantitative analysis of sprout length from PCAEC spheroids exposed to H/R. Treatment with DPI (10 μM), Apo (200 μm and 600 μM), Wortmannin (Wort; 500nM), or PD-98059 (25 μM) suppressed the H/R-stimulated increase in sprout length (n = 4 cell lines, data are means ± SD; *P < 0.05 compared with H/R; #P < 0.05 compared with baseline). C: images of WT and p47phox−/− MHMEC spheroids exposed to H/R for 24 h. D: quantitative analysis of sprout length from WT (white bars) and p47phox−/− (black bars) MHMEC spheroids exposed to H/R. H/R was less effective in stimulating spheroid spourting in p47phox−/− MHMECs compared with WT MHMECs (n = 3 cell lines, data are means ± SD, *P < 0.05 compared with H/R; #P < 0.05 compared with non-H/R).