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. 2008 Apr 22;9:20. doi: 10.1186/1471-2121-9-20

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Copyright © 2008 Cheng et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of stem cell markers. mRNA isolated from ChDPSCs was used for RT-PCR analysis. The expression of stem cell (Nanog, Rex-1, Oct-4) and differentiation (Osteonectin, Osteocalcin, Osteopontin) markers was detected. Bone sialoprotein was not detected in ChDPSCs. Internal control: GAPDH. Negative control: water. All primers were designed to cross the junction between two exons with the upstream primer located at the 3'region of the leading exon; whereas, the down stream primer was located at the 5'region of the following exon in order to minimize the possibility of a false positive or contamination of genomic DNA.