Table 1.
Name | Primer sequence | Amplicon length (bp) | Nucleotide (ORF)/amino acid position | |
Region 1 | Fb | 5' CCGTGGCCGAATACACCTT 3' | 127 | -167 |
R | 5' CATTGGCGCCCTTTACGTA 3' | |||
Region 2 | F | 5' GAGCTTCACCACAGCAAGCA 3' | 127 | 76–202/26–67 |
R | 5' AAGCTAGATTCTTTTCGTTCAGCAA 3' | |||
Region 3 | F | 5' CGTCGCCAAACTCGAAGAG 3' | 151 | 129–279/44–93 |
R | 5' GCCGGTGGGCTTGTCA 3' | |||
Region 4 | F | 5' GCCACGTCAATCACACCATCT 3' | 232 | 215–446/73–149 |
R | 5' AAGTTCGTCTGGTGGTCGTAAAC 3' | |||
Region 5 | F | 5' GGGACACACTCGGCAACAA 3' | 158 | 389–546/131–182 |
R | 5' CAAAACGCCTTGGCAAAGTC 3' | |||
Region 6 | F | 5' TTTACGACCACCAGACGAACTTC 3' | 161 | 425–585/150–195 |
R | 5' CGCATACCGTGACTGCACAT 3' |
aBased on M. tuberculosis H37Rv sodA sequence (GenBank no. AF061030)
bF – forward, R – reverse