Figure 8.

Redirected CTL assay against RPMI following mixed culture conditions following addition of 17-AAG. Normal donor PBMCs were primed using UV-irradiated (120 mJ/cm²) or mitomycin-treated (100 μg/mL) RPMI 8226 MM cells as whole cell immunogens for 28 days in 'mixed-culture conditions'. Non-primed, mito-primed, or UV-primed and PBMCs (effector cells) were then co-cultured with viable ³H-TdR-labeled RPMI 8226 MM cells (target cells) in a re-directed CTL assay. 17-AAG was added at 10 uM to the UV-irradiated RPMI cells for four hours and then washed away before the cells were added to the CTL culture. A standard ³H-TdR cytotoxicity assays was performed at E:T ratio of 50:1. Experiments were performed triplicate and expressed as the mean ± 2SEM.