Table 3.
Proteins involved in synthesis of nucleic acids, porphyrins, isoprenoids, vitamins and fatty acids that were detected and proposed to be wheat amyloplast constituents1.
| Nucleic acid synthesis | |
| General | |
| 75 Inorganic diphosphatase EC 3.6.1.1 | 2(ch, ot), 3(ch), 4 |
| 76 Quinolinate synthase A EC not available | 2(ch), 3(ch), 4 |
| 77 Nucleoside diphosphate kinase EC 2.7.4.6 | 2(ch, cy), 3(ch), 4 |
| Purines | |
| 78 Adenylate kinase A EC 2.7.4.3 | 2(ch, cy), 3(ch), 4 |
| 79 Adenylosuccinate synthetase EC 6.3.4.4 | 1,2(ch), 3(ch, mi), 4 |
| 80 Phosphoribosylaminoimidazolecarboxamide formyltransferase EC 2.1.2.3 | 2(ch, mi, nu), 3(ch), 4 |
| 81 Phosphoribosylformylglycinamidine cyclo-ligase EC 6.3.3.1 | 2(ch, cy, mi, pl), 3(ch), 4 |
| 82 Phosphoribosylaminoimidazolesuccinocarboxamide synthase EC 6.3.2.6 | |
| Pyrimidines | |
| 83 Aspartate carbamoyltransferase EC 2.1.3.2 | 1, 2(ch), 3(ch, mi), 4 |
| 84 Carbamoyl phosphate synthetase, large subunit EC 6.3.5.5 | 2(ch, cy), 3(ch), 4 |
| 85 Carbamoyl phosphate synthase, small subunit, glutamine dependent form EC 6.3.5.5 | 2(ch, cy), 3(ch), 4 |
| 86 Dihydroorotate dehydrogenase EC 1.3.99.11 | 2(ch, cy), 3(ch), 4 |
| Porphyrin synthesis | |
| 87 Coproporphyrinogen oxidase EC 1.3.3.3 | 2(ch), 3(ch), 4 |
| 88 Ferritin (no EC number) | 2(ch), 3(ch, mi), 4 |
| 89 Ferrochelatase EC 4.99.1.1 | 2(ch, mi), 3(ch), 4 |
| 90 Glutamate-1-semialdehyde 2,1-aminomutase EC 5.4.3.8 | 2(ch), 3(ch), 4 |
| 91 Heme oxygenase 1 EC 1.14.99.3 | 3(ch), 4 |
| 92 Hydroxymethylbilane synthase EC 4.3.1.8 | 2(ch), 3(ch), 4 |
| 93 Porphobilinogen synthase EC 4.2.1.24 | 2(ch, cy, pl), 3(ch), 4 |
| 94 Uroporphyrinogen decarboxylase EC 4.1.1.37 | 2(ch, pl), 3(ch), 4 |
| Isoprenoids | |
| 95 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase EC 2.7.7.60 | 2(ch, pl), 3(ch), 4 |
| 96 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase EC 1.17.4.3 | 2(ch), 3(ch), 4 |
| 97 Isopentenyl diphosphate DELTA DELTA 2 isomerase EC 5.3.3.2 | 2(ch, mi, pe, pl), 3(ch), 4 |
| 98 Phytoene synthase EC 2.5.1.32 | 2(ch, pl), 3(ch), 4 |
| 99 Phytoene desaturase EC 1.14.99.3? | 3(ch),4 |
| Vitamins and cofactors | |
| 100 Pyridoxamine 5'-phosphate oxidase-related domain containing protein EC 1.4.3.5 (related) | 3(ch, mi), 4 |
| 101 Riboflavin synthase, alpha chain EC 2.5.1.9 | 3(ch), 3(ch), 4 |
| 102 Thiamine biosynthesis protein ThiC EC not available | 2(ch), 3(ch), 4 |
| 103 Tocopherol cyclase EC not available | 2(ch), 3(ch), 4 |
| 104 Tocopherol methyltransferase EC 2.1.1.95 | 2(ch), 3(ch), 4 |
| Fatty acid synthesis | |
| 105 Acetyl-CoA carboxylase EC 6.4.1.2 | 2(ch, cy, mi, pl), 3(ch), 4 |
| 106 Acyl-[acyl-carrier-protein] desaturase EC 1.14.19.2 | 2(ch, cy), 3(ch), 4 |
| 107 [acyl-carrier-protein] S-malonyltransferase EC 2.3.1.39 | 2(ch, pl), 3(ch), 4 |
| 108 Beta-ketoacyl-acyl-carrier-protein synthase I EC 2.3.1.41 | 2(ch, mi), 3(ch), 4 |
| 109 Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) EC 1.3.1.10 | 2(ch), 3(ch), 4 |
| 110 3-Hydroxydecanoyl-[acyl-carrier-protein] dehydratase EC 4.2.1.60 | 2(ch), 3(ch), 4 |
| 111 Oleoyl-[acyl-carrier-protein] hydrolase EC 3.1.2.14 | 2(ch), 3(ot, ch),4 |
| 112 3-Oxoacyl-[acyl-carrier-protein] reductase EC 1.1.1.100 | 2(ch), 3(ch), 4 |
1Criteria: as in Table 1.